Ren has a calm demeanor and will speak up whenever her sister has difficulty expressing herself. While calm in nature like her father Zero Kiryu, Ren does not appear to have Zero's stoicism as she is shown smiling and joking around with Ai Kuran. However, while she seems to have inherited her playful side from her mother Yuki Kuran, her blunt nature definitely resembles a teenage Zero as she states whatever Ai has difficulty expressing. Ren also inherited the kindness of both her parents, never wishing harm on others. Ren's kindness is best exampled by staying at the Moon Dormitory instead of her home to keep distance from Ai when she craved her sister's blood. Unlike Ai, Ren knows what a "job" is, even explaining what one generally is; the topic came up when Kaname Kuran considered getting one, until Ren informed him that the Kuran family has wealth and resources. Ren knows about this due to attending Day Class in her childhood. Thanks to this, Ren also has experience cooking, as opposed to her sister. Even as a child, Ren showed a good deal of common sense, as she promised her mother to not reveal her true identity to her classmates or take energy from them in exchange for attending Day Class. Thanks to Ai doting on her as a child, Ren is very close to her older sister. Even as a child, Ren showed an interest in the role of protector as she would take the role of "knight" when she played dolls with Ai; she spent more time with Zero to learn how to use a gun, because she wanted to protected others with her own. When she got her fangs, Ren stayed away from Ai because she craved her blood (ironically enough) and the very thought of draining her dry scared her; she would dream of it, only worsening her self-hatred, as she had promised to protect her sister. She hates to remember this time in her life, because it was when she felt the most helpless.

The mother has long dark blonde hair tied in a low ponytail with her facial structure much like Zero and Ichiru's. She loves both her sons so much.

As a child, Ai was more mature for her age unlike Yuki Kuran. In the Extra "I Like You", she is shown to adore Zero Kiryu as her "Father", until the following 'Love's Desire' where she is shown to have romantic feelings for him. However, she keeps quiet for her mother's sake. Ai does, in fact, tell Ruka Souen her feelings. Ai was a very observant child and seemed more aware of things happening around her than the adults were aware of. Ai has a good sibling relationship with her sister Ren Kiryu as they are shown scolding one another. A young Ai was very much hyperactive in nature, a trait which has followed her into adulthood. Between the years of letting go of her crush on Zero and Ren's birth, Ai showed a rather dark side in trying to manipulate her mother and foster father into producing a sibling for her. Primarily so, Ai could love her sibling and drink their blood as she rather dislikes the alternatives of transfusion bag blood and blood tablets. Once Ren was born, Ai gave up on the idea, instead focusing more on being a loving sister. Ai is shown to not tolerate anyone insulting her beloved sister simply due to her not being a Pureblood. When an old woman still loyal to the old vampire society voiced disgust at the Kuran bloodline having a "filthy blooded" child, Ai snapped and used her Pureblood powers to silence her and threatened the old woman to never speak of her sister so disrespectfully. It was only thanks to Seiya and Ren intervening that Ai calmed down. Due to growing up in the lifestyle of a Pureblood, Ai is naïve of some lower class customs. For example, she did not know what a "job" was. Ai often became annoyed by letters from Pureblood families asking for her to marry a Pureblood lord. She ignored them constantly.

Juri was described as a cheerful and strong woman who puts her family first where she was mischievous and playful and enjoyed teasing Kaname Kuran. Even though Juri and Yuki had the same appearance, they have very different personalities. Juri was blunt, easily irritated by Haruka Kuran and sometimes loud and fierce when she lost her temper. She was very passionate about things that mattered to her as she vehemently objected to Kaien Cross's plan to kill all vampires, declaring that not all vampires were bad and physically opposed him. She was described by Isaya Shoto and Kaien as like the very blaze of the sun. Outspoken and high spirited, Juri was dedicated to her dreams of a peaceful existence with humans.

*Sara appeared to be a soft-spoken Pureblood among her fellow vampires who had a duplicitous nature, appearing refined in front of her peers while having secret goals of becoming the Queen of the vampires. Sara was very manipulative to the point of almost being sadistic, as shown with the schoolgirls she befriended. She referred to herself as impatient and was sadistic when she took pleasure in giving Takuma Ichijo's pain. She was determined not to fall prey to the Purebloods' habit of losing purpose, which was why she was making her bid to become Queen. She surrounded herself with servants and she seemed to enjoy their attention as they spoiled her.

Shizuka usually appeared as a calm and composed woman, who is always smiling as if amused when taunting or talking to Zero Kiryu. Her gentle movements gave her a striking presence as cherry blossoms often accompany her presence, even if they should be out of season, like when she first met the Kiryu twins. While possessing Maria Kurenai's body, Ichiru Kiryu notes that Shizuka's actions in Maria's body were different than her normal calm and composed demeanor, such as when she playfully toys around with Ichiru's feelings and when she interacts with the Night Class. This shows a bit of Shizuka's playful side, even though Ichiru seemed displeased with it. An example of this was when Shizuka (possessed in Maria's body) kissed Ichiru out of fun, which leads to Ichiru telling her not to do it again.

Seiren seems to be a no-nonsense type of girl, who can always be trusted to get her job done. She can seem cold, but is very loyal. She is a very quiet and enigmatic character who only speaks when necessary. Seiren may come across as harsh at first, and she can get irritated very quickly. Some say she can be a little too serious. Once Seiren befriends a person, she will defend them to her last breath. It is not wise to threaten her or others she knows, as she will kill without hesitation. As Hanabusa Aido points out, Seiren has never been known to lie.

Maria is a very fragile and feminine vampire. When she was first introduced, she was possessed by the Pureblood, Shizuka Hio and was displayed as haughty and cruel. However, her true personality is quite the opposite. She is very caring towards her friends and wishes to be seen as useful. Though she can show a competitive side when it comes to the Kiryu twins, she openly approaches Yuki Kuran about her relationship with Zero. Maria has no problems with discussing her feelings and is quite honest and straightforward. She still harbors feelings for Ichiru Kiryu and now shows interest in Zero Kiryu who ate his brother to become fully powered.

Ruka usually reflects as a haughty, bored, unapproachable and uninterested person to the rest of the world, probably due to her noble upbringing. She is portrayed to show disdain to those considered lower than her, or just simply ignoring them, refusing to acknowledge their presence. Ruka is sometimes cold to others affections, as shown when she rejects the Day Class Representatives' dance proposal by stating she cannot dance with someone she does not know. One of her defining characteristics is her love for Kaname Kuran as she has been tied by loyalty and admiration for him ever since they were children, angry at all those who disrespect him and disdainful of all girls who fawn on him. Her possessiveness made her hot-tempered and jealous, but her love for Kaname also brought out a vulnerable, self-doubting aspect of her usually proud personality, which she usually only expresses to Akatsuki Kain (Ruka's close friend). She later matures after she realizes that she was only thinking of her own feelings. Since giving up on him, she has shown a gentler side to her personality. She even gave Yuki Kuran, whom she was jealous of before, etiquette lessons and during the ball where she told her that she was glad Yuki and Kaname were getting along so well. Akatsuki, who is her cousin, has been in love with her since they were children. She seems to recognize his feelings for her later on after he pulls her aside and confesses his romantic interest in her, albeit she was surprised by his bold confession.

Rima possesses a strong and self-assured personality similar to Senri Shiki as she tends to expend energy only when necessary, and is usually a lady of few words. Normally, she comes across as a quick thinker, indifferent and aloof, but is often seen to be worrying about Senri and Takuma Ichijo. Despite her uninterested air she has no qualms speaking her mind and unexpectedly has a short-tempered side to her as well. She usually has an indifferent or disinterested expression on her face. As a vampire, she gets sunburned easily and can be seen walking under a parasol with Senri during the day. Cool, professional and occasionally cynical, Rima is also very loyal and protective of her friends.

Yori seems to be one of the few girls who isn't interested in the Night Class. She finds them a bit scary and stated that she would prefer someone from the Day Class. Yori is also very observant, as she notices a lot of things that happen to Yuki Kuran, especially when she gets hurt or is very depressed. Yori has a calm and serious personality as well. She has not shown fear when in the presence of vampires, especially those who hunger for her blood, and is indifferent to the fact that Yuki is a Pureblood. Even when Yori is married to Hanabusa Aido, who would drink her blood, Sayori was never afraid of him; she even noted he drank her blood, he did his utmost to be gentle. When she revealed her feelings to Hanabusa, she explained that she wasn't infatuated with him because of his looks like the Day Class girls were; it was because she saw the kind of person he was underneath his smugness and insecure moments. Though Yori did voice annoyance at Hanabusa putting off their marriage because Yuki and Zero hadn't; his "loyal dog" complex was one of things she found annoying. As Hanabusa would note, Yori is strong willed. She refused to be turned into a vampire even if it meant dying long before her friends. Though she briefly considered asking Yuki to turn her into a vampire, shortly after the birth of her son; the realization that she would die and leave her family saddened had shaken her resolve. In the end she refused to give up her humanity; even when her son begged Yori to do it, just to live long enough for Hanabusa to make the cure and age so they could have their twilight years together. Yori had enough foresight to ask her grandchildren to befriend Yuki after her death, to keep her from being lonely.

Yuki was generally a cheerful, caring and a rather comedic girl with her beloved smile. She was considered a poor student because she dislikes studying and falls asleep in class due to the fact that she stays up all night monitoring the school grounds and protecting the Day Class students from sneaking into the Night Class students. She exhibits a strong attachment to her close friends, especially Sayori Wakaba who Yuki affectionately calls "Yori" and Zero Kiryu. Her indecisive nature and reticence can be attributed to her insecurity regarding her missing past. As a human, she has been seen as standing sides with vampires, but deep in her heart she feared all vampires (because of a vampire that attacked her when she was little) excluding Kaname Kuran. Due to this fear, she rarely left the Cross Academy grounds on her own. Yuki doesn't like to reveal her fears and disguises it with a smile. Zero, however, having grown up with her for four years, was always able to tell when something is bothering her, making it hard for her to hide things from him and vice versa. Following her reawakening as a Pureblood vampire, Yuki shed the fears she once retained. When a situation demands for it, she can put in an authoritative face as expected of a Pureblood Princess. As a result, she has grown more emotionally mature and thoughtful. Yuki's overwhelming fault is the tendency to burden herself with guilt over her ignorance when she learns of other people's sufferings that they have kept secret. Yuki's favorite subject was Physical Education and her favorite food was ginger pork stir fry set, as well as parfaits.

Sharma is an Indian vampire and former general associated with Dracula's forces. He is a dark-skinned man who wears traditional Indian-inspired clothing and is often seen alongside Raman, another Indian vampire from the same group. The two share similar features and frequently appear together. Sharma is an agile and exceptionally fast fighter who specializes in dual scimitars. His fighting style relies on speed, precision, reflexes, and graceful movement rather than brute force. He is confident in his abilities and remains composed when facing skilled opponents. Sharma is best remembered for his distinctive appearance, Indian heritage, elegant fighting style, and close association with Raman. His combination of speed, athleticism, and swordsmanship makes him one of the more recognizable vampire warriors in Dracula's forces.

Growing up in Chō's vampire court, Taka and Sumi spent most of their lives experiencing how cruel vampires can be. Both are dedicated to killing vampires to ensure no one has to experience what they have again. Taka is the more headstrong of the duo. He can be tactless in his actions but is also daring. Sumi and Taka are eager to learn all Alucard has to teach, but they are impatient and eventually decide that he is standing in their way of learning all the secrets. Secretly, both are traumatized by their difficult lives and don't trust anyone. As soon as they realized Alucard was also not to be trusted, they chose to take his holdings for themselves. It is implied that Sumi and Taka secretly want personal power to build their own empire. They are consistently seeking to learn the castle's teleportation magic for this reason. They withheld their real intent and justify their attempt at Alucard's life by accusing him of being the one who withheld information from them.

Ratko is a highly skilled and intimidating warrior known for his confidence, discipline, and expertise in strategy. He considers himself an expert in military tactics and takes great pride in understanding how battles work. Proud and outspoken, Ratko dislikes being underestimated and becomes particularly irritated when others question his abilities. Despite his reputation, Ratko often finds himself working under powerful leaders whose talents he does not always respect. He dislikes having to restrain his personality or hide his intelligence simply to satisfy those above him. Ratko would much rather operate independently, where he can demonstrate his full potential. He especially enjoys facing opponents who can challenge his abilities and respects people who possess genuine skill and determination. Beneath his aggressive confidence is a calculating and intelligent mind, making Ratko a dangerous strategist and an unpredictable adversary.

Death is an ancient and mysterious supernatural entity driven by an endless hunger for the life energy of others. He is cold, calculating, merciless, and almost completely lacking in empathy. Unlike ordinary beings, Death views life through an entirely detached perspective, seeing living creatures primarily as sources of energy that sustain his existence. His greatest desire is to overcome his eternal hunger and reach a state of absolute power. Death is patient, intelligent, manipulative, and exceptionally confident. He has little regard for morality or the feelings of others, making him one of the darkest and most dangerous beings in his world. He rarely shows genuine emotion and prefers to manipulate situations from the shadows. His overwhelming ambition, supernatural nature, and complete disregard for conventional morality make him an intimidating and enigmatic presence.

Varney is leading a gruesome siege in the city of Targoviste, which he’d hoped to conquer and gift to Dracula before the vampire lord’s untimely death. The city lies in ruins, with the human survivors slowly starving to death in the streets while the king and queen hide underground. Varney is winning but for whom? No one cares about him. But there’s more to Varney than what we see in the first couple of episodes of the season. Varney’s plan goes far beyond wiping out a city and killing Trevor Belmont and Sypha Belnades once and for all. He actually turns out to be the main villain, and the final boss, of the season.

Godbrand is a bold, energetic, and outspoken traveler with a larger-than-life personality. He enjoys adventure, exploration, good company, and sharing stories of his experiences. Confident and humorous, he often speaks before thinking and isn't afraid to voice his opinions. While he can appear reckless or stubborn, he is surprisingly observant and often notices details that others miss. Godbrand values freedom, independence, and living life on his own terms. He enjoys friendly competition, lively conversations, and finding excitement wherever he goes. Beneath his confident exterior is a practical thinker who understands people well and adapts quickly to changing situations. His combination of humor, charisma, and unpredictability makes him both entertaining and memorable to those around him.

Isaac is a determined and highly intelligent strategist from Castlevania. Deeply loyal to those he trusts, he values respect, honesty, and commitment above all else. Initially shaped by a difficult past and a cynical view of the world, Isaac spends much of his journey questioning his beliefs and searching for a greater purpose. Reserved, disciplined, and thoughtful, he approaches challenges with patience and careful planning rather than impulsiveness. His travels expose him to different people and perspectives, leading him to become more reflective and open-minded. Over time, Isaac grows from someone defined by loyalty to others into someone guided by his own principles and vision for the future. Despite his intimidating presence, he possesses a strong sense of responsibility and a surprising capacity for understanding and forgiveness. His story is one of personal growth, self-discovery, and learning how to build something meaningful rather than dwelling on the past.

Hector is a thoughtful and intelligent craftsman from Castlevania, known for his rare talents, curiosity, and love of learning. Quiet and reserved, he prefers studying, creating, and caring for the creatures under his protection rather than seeking power or conflict. Although often misunderstood by others, Hector is kind-hearted, empathetic, and deeply values friendship, loyalty, and compassion. Because he spent much of his life isolated from society, Hector can be trusting and somewhat naive, often believing the best in people even when they do not deserve it. However, his experiences gradually help him become wiser, more independent, and more confident in his own judgment. Despite facing many hardships, he remains resilient and refuses to lose his gentle nature. Hector's greatest strengths are his intelligence, creativity, patience, and capacity for forgiveness, making him one of the most compassionate and emotionally complex characters in the series.